B7542
Maleimide Activated BSA
Synonyme(s) :
Maleimide Activated Bovine Serum Albumin
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About This Item
Code UNSPSC :
12352111
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
bovine
Niveau de qualité
Stérilité
non-sterile
Forme
lyophilized powder
Technique(s)
ELISA: suitable
bioconjugation: suitable
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
bovine ... ALB(280717)
Description générale
Maleimide Activated BSA is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated BSA contains approximately 5-15 maleimide groups for each BSA molecule.. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent) to detect unreacted thiol groups in the cysteine-containing peptide.
Application
Maleimide Activated BSA has been used in peptide conjugation.
Maleimide-activated BSA (mBSA) has reactive maleimide groups on its surface available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.
Forme physique
Each vial contains 5 mg of Maleimide Activated BSA lyophilized from 10 mM sodium phosphate buffer, pH 6.6, with 0.115 mM NaCl, 1 mM EDTA, and 40 mM sucrose as stabilizer.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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