Accéder au contenu
MilliporeSigma

Principaux documents

Voir toute la documentation

Passer à

B0519

Biotin−Agarose

PBS suspension

Synonyme(s) :

Vitamin H Agarose

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue
À vous/RéférenceDisponibilitéPrix
5 mL
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
656,00 $
25 mL
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
2 570,00 $
2 184,50 $

A propos de cet article

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00163072

656,00 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
​
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

form

PBS suspension

Quality Segment

200

extent of labeling

≥30 mg avidin per mL packed resin, 2-6 μmol per mL

matrix

4% beaded agarose

matrix activation

epoxy

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix spacer

13 atoms

application(s)

life science and biopharma

storage temp.

2-8°C

Application

Biotin contains a high affinity for binding to avidin or streptavidin. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins.

Physical form

Suspension in phosphate buffered saline containing 0.02% sodium azide

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 1

Cet article
B0301S1638B1116000
Biotin−Agarose PBS suspension

Sigma-Aldrich

B0519

Biotin−Agarose

Biotine meets USP testing specifications

Sigma-Aldrich

B0301

Biotine

Streptavidin−Agarose from Streptomyces avidinii buffered aqueous suspension

Millipore

S1638

Streptavidin−Agarose from Streptomyces avidinii

Biotine European Pharmacopoeia (EP) Reference Standard

B1116000

Biotine

application(s)

life science and biopharma

application(s)

pharmaceutical (small molecule)

application(s)

-

application(s)

pharmaceutical (small molecule)

form

PBS suspension

form

powder

form

buffered aqueous suspension

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix attachment

-

matrix attachment

amino

matrix attachment

-

matrix

4% beaded agarose

matrix

-

matrix

4% beaded agarose

matrix

-

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Questions

1–3 of 3 Questions  

  1. Salve ho acquistato questo prodotto. Esiste un protocollo di massima per il suo utilizzo?

    1 answer

    1. Unfortunately, a standard protocol is not available for this product. The sample binding capacity protocol below may be helpful:
      1. Pipette 2.0 mls of resin into pre-calibrated, fritted columns and allow the resin to pack. Measure the packed volume in milliliters.
      2. Wash the resin with 5 packed volumes of 0.5 M sodium chloride.
      3. Equilibrate the resin with 25 ml packed volumes of phosphate buffered saline (PBS), product P4417. Check the pH of effluent after the wash. The pH should be 6.75 to 7.25. If necessary, equilibrate with additional 25 X packed volume.
      4. Dissolve avidin from egg white, product A9275 at 80 mg protein/ml in PBS. Check the pH and record. If necessary, adjust the pH to 6.95 to 7.05 with 0.1 N HCl.
      5. Determination of Avidin load:
      PBS 10.0 ml
      Avidin at 80 mg/ml 0.05 ml
      Mix by inversion and measure A (280 nm) versus PBS.
      6. Calculation of Avidin Load:
      [(Net Absorbance (280 nm) x 10. 05]/(0.05 x 1.54)
      7. For each ml of packed gel in the column, add 1.0 ml of the avidin solution. Allow the column gel to equilibrate in the presence of the avidin for one hour with intermittent swirling.
      8. Wash the column with 100 packed volumes of PBS. Mix each wash by inversion and measure A (280nm) versus PBS.
      Calculation of wash:
      [(Net Absorbance (280nm) x 100] / (1.54)
      9. Bound avidin to biotin agarose= mg of avidin labeled - total avidin in wash (unbound).
      10. Binding capacity = (Bound avidin to biotin-agarose)/ml of packed gel.

      Additional references and general protocols are also available at the links provided below:
      https://www.sigmaaldrich.com/search/b0519?focus=papers&page=1&perpage=30&sort=relevance&term=B0519&type=citation_search
      https://www.sigmaaldrich.com/applications/protein-biology/protein-pulldown

      Helpful?

  2. Could you provide the protocol for this product?

    Could you provide the protocol for this product?

    1 answer

    1. Please refer to the following literature for guidance:
      - https://www.nature.com/articles/srep42849
      - https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btpr.2293

      Helpful?

  3. Is there a protocol available for this product?

    1 answer

    1. Unfortunately, there is no in-house protocol available for this product.

      Helpful?

Reviews

No rating value

Active Filters