A0157
Ascorbate Oxidase from Cucurbita sp.
lyophilized powder, 1,000-3,000 units/mg protein
Synonyme(s) :
L-Ascorbate:oxygen oxidoreductase, Ascorbase
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About This Item
Produits recommandés
Source biologique
plant (Cucurbita spp.)
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
1,000-3,000 units/mg protein
Température de stockage
2-8°C
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Description générale
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Application
Ascorbate Oxidase from Cucurbita sp. has been used:
- as a supplement in the culture medium for differentiation into osteoblasts
- to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
- in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis .
Actions biochimiques/physiologiques
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.
Définition de l'unité
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.
Forme physique
Lyophilized powder containing buffers and sucrose as stabilizer.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
Cotton ascorbate oxidase promotes cell growth in cultured tobacco bright yellow-2 cells through generation of apoplast oxidation
Li R, et al.
International Journal of Molecular Sciences, 18(7), 1346-1346 (2017)
Manuel A Matamoros et al.
Journal of experimental botany, 61(1), 87-97 (2009-10-14)
In plant cells, antioxidants keep reactive oxygen species at low concentrations, avoiding oxidative damage while allowing them to play crucial functions in signal transduction. However, little is known about the role of antioxidants during fruit maturation, especially in legumes. Snap
Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Sewelam N, et al.
PLoS ONE, 8(8), e70289-e70289 (2013)
Cécile Garchery et al.
Plant, cell & environment, 36(1), 159-175 (2012-06-26)
The regulation of carbon allocation between photosynthetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool. RNA
A Messerschmidt et al.
Journal of molecular biology, 224(1), 179-205 (1992-03-05)
The crystal structure of the fully oxidized form of ascorbate oxidase (EC 1.10.3.3) from Zucchini has been refined at 1.90 A (1 A = 0.1 nm) resolution, using an energy-restrained least-squares refinement procedure. The refined model, which includes 8764 protein
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