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70168

Millipore

Ox-bile, dehydrated, purified

suitable for microbiology, extracted under controlled conditions from purified fresh bile

Synonyme(s) :

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

Code UNSPSC :
41106212
Nomenclature NACRES :
NA.85

Niveau de qualité

100

Stérilité

non-sterile

Forme

powder

Impuretés

~5% sodium chloride

Résidus de calcination

≤22% (as SO4)

Perte

≤8% loss on drying

pH

6.0-9.2 (2% in H2O)

Solubilité

distilled water: freely soluble

Application(s)

microbiology

Catégories apparentées

Description générale

Ox-bileis a dehydrated, purified fresh bile which is used as a selective inhibitory agent in bile media. Ox-bile desiccated is prepared by a low temperature dehydration process which ensures a uniform product and eliminates the difficulties encountered in obtaining satisfactory supplies of fresh bile. An 8 to 10% solution of the dehydrated product is equivalent to fresh bile. 1 gram of dried Ox Bile is equivalent to 8 grams of fresh bile.

Application

Ox bile is used as a selective agent in culture media for selective growth of intestinal bacteria like E. coli, other feacal coliforms, feacal streptococci. But it is also used as a selective agent in media for detection of Yersinia enterocolitica, Aeromonas hydrophila and Vibrio species. Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio vulnificus (Beneckea) which is a halophilic bacterium, found responsible for causing several human infections.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCM4509
BCCJ9775
BCCK4876
BCCK8259
BCCG3744

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Construction of a probiotic lactic acid bacterium that expresses acid-resistant phytase enzyme
MAJIDZADEH HR, et al.
Journal of Agriculture, Science and Technology, 18(1), 925-936 (2016)
Molecular and Probiotic Characterizations of Lactobacillus reuteri DSM 12246 and Impact of pH on Biomass and Metabolic Profile in Batch-Culture
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Survival of Mycobacterium bovis BCG oral vaccine during transit through a dynamic in vitro model simulating the upper gastrointestinal tract of badgers
Williams GA, et al.
PLoS ONE, 14(4), e0214859-e0214859 (2019)
Gareth A Williams et al.
PloS one, 14(4), e0214859-e0214859 (2019-04-20)
In developing an oral bait BCG vaccine against tuberculosis in badgers we wanted to understand the conditions of the gastrointestinal tract and their impact on vaccine viability. Conditions mimicking stomach and small-intestine caused substantial reduction in BCG viability. We performed
Functional properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
Maldonado N, et al.
International Journal of Veterinary Science & Research, 1(1), 011-022 (2015)
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