PMSF-RO
Roche
Fluorure de phénylméthanesulfonyle
Phenylmethylsulfonyl fluoride
Synonyme(s) :
αFluorure d'α-toluènesulfonyle, Fluorure de benzylsulfonyle, Fluorure de phénylméthylsulfonyle, PMSF
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About This Item
Formule empirique (notation de Hill):
C7H7FO2S
Numéro CAS:
Poids moléculaire :
174.19
Numéro Beilstein :
2088311
Numéro MDL:
Code UNSPSC :
12352202
ID de substance PubChem :
Produits recommandés
Niveau de qualité
Pureté
98% (TLC)
Forme
solid
Poids mol.
174.2
Conditionnement
pkg of 10 g (10837091001)
pkg of 25 g (11359061001)
Fabricant/nom de marque
Roche
Concentration
17-170 μg/mL
Technique(s)
western blot: suitable
pKa
7.0
Pf
92-94 °C
Solubilité
>10 mg/mL
Conditions d'expédition
ambient
Température de stockage
20-25°C
Chaîne SMILES
FS(=O)(=O)Cc1ccccc1
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
Clé InChI
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
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Catégories apparentées
Description générale
Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.
Spécificité
Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
Application
PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
Actions biochimiques/physiologiques
PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.
Qualité
Purity: Chromatographically homogeneous
Notes préparatoires
Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Reconstitution
Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.
Autres remarques
Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
For life science research only. Not for use in diagnostic procedures.
Certificats d'analyse (COA)
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