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UFC9100

Millipore

Filtre à centrifuger Amicon® Ultra, MWCO de 100 kDa

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa

Synonyme(s) :

Concentrateur centrifuge, Seuil de coupure de 100 kDa, Volume d'échantillon de 15 ml

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8 PKG
166,00 $
24 PKG
435,00 $
96 PKG
1 690,00 $

166,00 $

Date d'expédition estimée le21 avril 2025Détails

Devis pour commande en gros
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Sélectionner une taille de conditionnement

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8 PKG
166,00 $
24 PKG
435,00 $
96 PKG
1 690,00 $

About This Item

Code UNSPSC :
41104916
eCl@ss :
36100101
Nomenclature NACRES :
NB.24

166,00 $

Date d'expédition estimée le21 avril 2025Détails

Devis pour commande en gros

Matériaux

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Niveau de qualité

400

Stérilité

non-sterile

Gamme de produits

Amicon®

Conditionnement

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

Fabricant/nom de marque

Amicon® Ultra

sustainability

Greener Alternative Product

Paramètres

15 mL sample volume

Technique(s)

protein extraction: suitable
protein purification: suitable

Longueur

12.1 cm (4.807 in.)

Diamètre

2.97 cm

Surface de filtration

7.6 cm2

Volume

15 mL

Volume de travail

≤15 mL
15 mL

Dimension de pores

100 kDa MWCO

Autre catégorie plus écologique

Re-engineered

Conditions d'expédition

ambient

Catégories apparentées

Description générale

Les filtres à centrifuger Amicon® Ultra de 15 ml avec membrane Ultracel® à seuil de coupure de 100 000 Da assurent une ultrafiltration rapide et une rétention fiable des macromolécules. Par exemple, l'IgG (156 kDa) en concentration initiale de 1 mg/ml est retenue à plus de 90 %.

Leur configuration verticale et leur surface utile de filtration en font des dispositifs très performants : traitement rapide, très bon taux de récupération (en général plus de 90 % de la solution de départ diluée) et possibilité de concentrer l'échantillon 80 ×. Leur configuration verticale permet en outre de minimiser la polarisation du soluté, et donc, le colmatage de la membrane qui en découle. Enfin, la présence d'une butée physique à l'intérieur du dispositif empêche d'atteindre la siccité tout en prévenant les éventuelles pertes d'échantillon.

Le concentré est récupéré dans le réservoir à échantillon du filtre à l'aide d'une pipette, tandis que l'ultrafiltrat est recueilli dans le tube à centrifuger fourni. Le filtre peut être centrifugé dans un rotor à godets oscillants ou à angle fixe. Avec leur membrane verticale et leur butée fixe, les filtres à centrifuger Amicon® Ultra de 15 ml offrent un taux de récupération maximal pour un temps de centrifugation relativement court.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Application

  • Ultrafiltration sur membrane Ultracel® à seuil de coupure de 100 000 Da
  • Concentration d'échantillons biologiques contenant des antigènes, des anticorps, des enzymes, des acides nucléiques ou des micro-organismes
  • Purification des composants macromoléculaires présents dans des extraits de culture tissulaire ou des lysats cellulaires et élimination des protéines avant chromatographie liquide haute performance
  • Concentration de protéines diluées ou prépurifiées à partir d'éluants de colonne
  • Dessalage, échange de tampon et dialyse des protéines

Caractéristiques et avantages

  • Membrane Ultracel® en cellulose régénérée à seuil de coupure nominal de 100 kDa offrant une récupération du rétentat supérieure à 90 %
  • Réduction de la polarisation de la concentration grâce à la membrane verticale, pour des temps de centrifugation ultrarapides (à peine 10 à 15 minutes)
  • Membrane thermoscellée minimisant les quantités de matières extractibles en aval
  • Tests d'intégrité réalisés sur 100 % des filtres pour garantir la fiabilité des résultats
  • Visualisation pratique de l'échantillon grâce au tube translucide et aux graduations de volume
  • Pipetage direct de l'échantillon (suppression de l'étape de récupération du concentré)
  • Facteurs de concentration élevés (80 × à 100 ×)

Informations légales

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Ultrafiltration de nanoparticules

Méthodes de filtration et sélection d'un filtre membrane pour la purification et la production de nanoparticules.

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

Questions

1–10 of 13 Questions  

  1. Is it reusable? How many times I can fill it up again? Amicon Ultra 15. First time I fill it up with 15 ml, and after can I use it again for other 15 ml....and again?

    1 answer

    1. The Amicon Ultra 15 is designed for single use but can be used for repeated spins with the same sample. However, repeated use will cause clogging of the membrane, leading to slower flow rates and longer spin times.

      Helpful?

  2. Hello, Are these tubes valid for AAV concentration? And if so, are these valid for all serotypes? Also, the user guide says that the membrane's material is 'Ultracel® low binding regenerated cellulose'. What's the difference with the PES membrane?

    1 answer

    1. These devices can be used for virus concentration but are not validated for any specific type. Both PES and regenerated cellulose membranes have low binding, but PES has a faster flow rate. For more information, please refer to the technical article on the website: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/viral-concentration-amicon-ultrafiltration

      Helpful?

  3. Hi, I have plan to use the Amicon Ultra-15 100K for Exosome extraction from cell culture supernatant, could you please let me know what would be the best speed (rpm/rcf) and time for doing that? Thanks

    1 answer

    1. Typically, Amicon-15 devices are spun at 4,000 x g (relative centrifugal force) for exosome isolation. However, spin times may need to be optimized, based on the sample type and volume. The image below demonstrated the relationship between volume and spin times. Please see the link below to review the product User Guide. More specific spin time information is available on page 3.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      Helpful?

  4. What is the minimum starting volume I can load on Ultra 100K (100,000 MWCO) devices? Can I load as little as 500 µL?

    1 answer

    1. This product is intended for use with 4-15 mL initial sample volumes. The device has not been evaluated for use with volumes smaller than those recommended. While it may be possible to concentrate samples as low as 500 uL, the recovery volume cannot be predicted. Please see the link below to review an alternative Amicon device specifically designed for 500 uL samples.
      https://www.sigmaaldrich.com/product/mm/ufc5100

      Please see the following link to review the full list of 100 kDa MWCO Amicon Ultra products:
      https://www.sigmaaldrich.com/substance/amiconultracentrifugalfilter100kdamwco1234598765

      Helpful?

  5. Does the Amicon Ultra has residues that may released into the concentrated product', like Beta-Glucan?

    1 answer

    1. These devices contain trace amounts of glycerine. If this material interferes with downstream analysis, rinse the device with buffer or Milli-Q® water prior to use. Should interference continue, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water. NOTE: Do not allow the membrane to dry out following rinse steps. If the product will not be used immediately after rinsing, leave the final buffer or water on the membrane until the usage. Please see the link below to review the full product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      Helpful?

  6. I just noticed that Amicon ultra - 15 centrifugal filters are non-sterile. Has this been always the case? REF: UFC910024, LOT:0000193199

    1 answer

    1. The Amicon Ultra devices have always been non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.

      Helpful?

  7. What is the maximum volume that can be loaded into the Amicon 15 device when using both fixed angle and swinging bucket rotor centrifuges?

    1 answer

    1. When using the fixed angle centrifuges with the Amicon 15 device, the maximum volume that can be loaded is 12 mL. It is important to place the device at a 35-degree angle. On the other hand, when using swinging bucket centrifuges, the maximum volume that can be loaded into the Amicon 15 device is 15 mL.

      Helpful?

  8. Hi, how should we place the amicon ultra-15 tube in the fixed-angle rotor, (could you please explain the sentence "When using a fixed-angle rotor, orient the device with the membrane panel facing up" , sorry I'm not a native English speaker

    1 answer

    1. When using a fixed-angle rotor, orient the device with the membrane panel facing up as demonstrated in the image below.

      For a more detailed view, kindly review the attached video link: https://youtu.be/GTH7kQMUjFg?feature=shared&t=145. This video also discusses the relative size of each Amicon® Ultra Centrifugal Filter and what kind of rotors they will fit into.

      Helpful?

  9. Hii, I wold like use these tubes for ultrafiltration of FBS to deplete it from EVs. I found they were used in one publication for such an experiment. How can they be sterilized so they can be used in cell culture hoods?

    1 answer

    1. Amicon Ultra devices cannot be sterilized by UV or autoclaving. These products are intended as a 1 time use device, however it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).

      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.

      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).

      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.

      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.

      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.

      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from the device as previously described prior to use.

      Helpful?

  10. Hi, Can you please confirm the centrifugation speeds ( in rcf ) for the Amicon-15 100kDa and the 10kDa filters ? I was told that the max speed for the 100kDa is 3000g and for the 10kDa is 4000g, respectively. Is that the case and if so, why exactly ?

    1 answer

    1. Spin times range from 10 - 40 minutes and will depend on the sample types as well as the rotor (fixed-angle or swinging bucket). See the image below for spin times and speeds for each rotor type.
      Please see the link below to review the product FAQs for additional usage information:
      https://www.emdmillipore.com/US/en/product/Amicon-Ultra-15-Centrifugal-Filter-Unit,MM_NF-UFC910008#documentation

      To request the product user guide, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Documentation" under the Products Section with all the required information so that a member of our team can reach out to you to assist further.

      Helpful?

1–10 of 13 Questions  

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