SCC119
ImKC Immortalized Mouse Kupffer Cell Line
The ImKC Murine Immortalized Kupffer Cell Line can be used to develop in vitro model cell systems to advance the study of liver injury, function, and disease.
Synonyme(s) :
ImKC Cell Line
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.81
Produits recommandés
Source biologique
mouse
Technique(s)
cell based assay: suitable
cell culture | mammalian: suitable
Conditions d'expédition
ambient
Description générale
ImKC is a cytokine-producing immortalized Kupffer cell line that was established from transgenic mice expressing the thermolabile mutant tsA58 of the Simian virus 40 large T antigen under the control of the H-2kb promoter. Primary KC were obtained using a three step procedure: liver perfusion, centrifugal elutriation, and sorting for F4/80+ cells. ImKC were identified within the small-intermediate population of KC that maintained stable expression of F4/80, and the surface antigens CD11b, CD14 and TLR4.
Kupffer cells (KC), also known as stellate macrophages, are liver macrophages that reside in the lumen of the liver sinusoids. Kupffer cells play a critical role in maintaining liver functions including the removal of pathogens & foreign materials, antigen presentation and modulating innate immune responses. In vitro studies of Kupffer cells have been hindered by the difficulty in isolating sufficient number of primary cells and the limited lifespan of the cells in culture.
Description de la lignée cellulaire
Macrophage Cells
Application
Research Category
Inflammation & Immunology
Metabolism
Inflammation & Immunology
Metabolism
Research Sub Category
Liver Physiology
Liver Physiology
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Qualité
• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination
Stockage et stabilité
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Julia Rudolph et al.
Scientific reports, 11(1), 15702-15702 (2021-08-05)
Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial
Hepatocyte SREBP signaling mediates clock communication within the liver.
Guan, et al.
The Journal of Clinical Investigation, 133 (2023)
Eui Jin Kim et al.
Pharmaceutics, 13(3) (2021-04-04)
Inflammation is a defense mechanism that protects the body from infections. However, chronic inflammation causes damage to body tissues. Thus, controlling inflammation and investigating anti-inflammatory mechanisms are keys to preventing and treating inflammatory diseases, such as sepsis and rheumatoid arthritis.
Nicholas J Lennemann et al.
Journal of virology, 95(18), e0107321-e0107321 (2021-07-08)
Sudan virus (SUDV) is one of five filoviruses that compose the genus Ebolavirus that has been responsible for episodic outbreaks in Central Africa. While the SUDV glycoprotein (GP) structure has been solved, GP residues that affect SUDV entry have not
Mincle-GSDMD-mediated release of IL-1I? small extracellular vesicles from hepatic macrophages in ethanol-induced liver injury.
Zhang, et al.
Hepatology communications, 7 (2023)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique