MABF2032M

Anti-CD31 (PECAM-1) Antibody, clone MBC 78.1

clone MBC 78.1, from mouse

• Synonyme(s) : Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM. GPIIA′, PECA1, CD31
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: MBC 78.1, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_000433.4
• Numéro d'accès UniProt: P16284
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PECAM1(5175)

Description

Description générale

Platelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA′, PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting ′detachment′ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.

Spécificité

Clone MBC 78.1 detects CD31/PECAM-1 in human cells. It targets an epitope within the IgG homology domain 5.

Immunogène

PECAM-1 purified from human platelets.

Application

Anti-CD31 (PECAM-1), clone MBC 78.1, Cat. No. MABF2032, is a mouse monoclonal antibody that detects CD31/PECAM-1 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Immunoprecipitation.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CD31 (PECAM-1) in THP-1 cells.

Immunoprecipitation Analysis: A representative lot detected CD31 (PECAM-1) in Immunoprecipitation applications (DeLisser, H.M., et. al. (1994). J Cell Biol. 124(1-2):195-203).

Flow Cytometry Analysis: A representative lot detected CD31 (PECAM-1) in Flow Cytometry applications (DeLisser, H.M., et. al. (1994). J Cell Biol. 124(1-2):195-203).

Flow Cytometry Analysis: 1 µg from a representative lot detected CD31 (PECAM-1) in one millioin THP-1 cells.

Research Category
Inflammation & Immunology

Qualité

Evaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31 (PECAM-1) in Jurkat cells.

Description de la cible

82.54 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1