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MABC006

Sigma-Aldrich

Mouse IgG2b Negative Control, clone GC198

Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.42

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Clone

GC198, monoclonal

Fabricant/nom de marque

Chemicon®

Concentration

0.5 mg/mL

Technique(s)

flow cytometry: suitable
immunofluorescence: suitable

Isotype

IgG2b

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Spécificité

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Immunogène

Protein I preparation from Neisseria gonorrhoeae.

Application

Mouse IgG2b is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Liaison

Replaces: CBL602

Forme physique

Format: Purified
Purified immunoglobulin. The antibody is supplied in 0.5ml 25mM Tris-HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Stockage et stabilité

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Kristina Hopfensperger et al.
mBio, 11(4) (2020-07-16)
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Wells S Brown et al.
Cell reports. Medicine, 1(8), 100131-100131 (2020-12-10)
Activating KRAS mutations are found in over 90% of pancreatic ductal adenocarcinomas (PDACs), yet KRAS has remained a difficult target to inhibit pharmacologically. Here, we demonstrate, using several human and mouse models of PDACs, rapid acquisition of tumor resistance in
Jennifer M Colón et al.
Journal of neurotrauma, 33(18), 1696-1708 (2016-02-21)
Spinal cord injury (SCI) is a condition with no available cure. The initial physical impact triggers a cascade of molecular and cellular events that generate a nonpermissive environment for cell survival and axonal regeneration. Spinal cord injured patients often arrive
Victoria Karakis et al.
The Journal of biological chemistry, 299(5), 104650-104650 (2023-03-28)
Human trophoblast stem cells (hTSCs) have emerged as a powerful tool to model early placental development in vitro. Analogous to the epithelial cytotrophoblast in the placenta, hTSCs can differentiate into cells of the extravillous trophoblast (EVT) lineage or the multinucleate syncytiotrophoblast
Joshua N Douglas et al.
Journal of neuroinflammation, 13(1), 178-178 (2016-07-09)
Neurodegeneration is believed to be the primary cause of permanent, long-term disability in patients with multiple sclerosis. The cause of neurodegeneration in multiple sclerosis appears to be multifactorial. One mechanism that has been implicated in the pathogenesis of neurodegeneration in

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