Yes, low-fluorescent PVDF membrane is required in Licor IR Imaging Systems. The Immobilon-FL PVDF Membrane is suitable for this purpose. Immobilon-FL Membrane is the first transfer membrane optimized for fluorescence applications, with extremely low background that improves the sensitivity of all fluorescence detection protocols. It is compatible with all commonly used fluorescent probes at all excitation and emission wavelengths, making it ideal for multiplexing and chemifluorescence applications.
IPFL07810
Immobilon®-FL PVDF Membrane
1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Synonyme(s) :
Western blotting membrane, blotting membrane, transfer membrane
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10 EA
140,00 $
140,00 $
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A propos de cet article
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane
plain filter
white filter
plain filter
white filter
Detection method:
chemiluminescent
colorimetric
fluorometric
colorimetric
fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
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Laissez-nous vous aiderNom du produit
Membrane de transfert Immobilon®-FL en PVDF, 0.45 μm pore size, 7 X 8.4 cm sheets
material
PVDF membrane
plain filter
white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable
western blot: suitable
filter L × W
7 cm × 8.4 cm
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
detection method
chemiluminescent
colorimetric
fluorometric
shipped in
ambient
Quality Level
1 of 4
Cet article | IPFL10100 | IPFL00010 | IPFL20200 |
|---|---|---|---|
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| compatibility for use with Amido black, for use with Coomassie brilliant blue, for use with CPTS, for use with Ponceau-S red | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red |
| detection method chemiluminescent, fluorometric, colorimetric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
Application
Features and Benefits
Membrane de transfert Immobilon®-FL :
- Conçue pour donner des résultats optimaux dans les applications d'immunodétection par fluorescence
- Le très faible bruit de fond améliore la sensibilité de tous les protocoles d'immunodétection par fluorescence
- Compatible avec toutes les sondes fluorescentes courantes sur une large plage de longueurs d'onde d'excitation et d'émission
- Destinée à la détection par chromogène ou par chimioluminescence classique
General description
La membrane de transfert Immobilon®-FL est une membrane en poly(fluorure de vinylidène) (PVDF) microporeuse conçue pour adsorber les protéines transférées à partir de diverses matrices de gel. La membrane Immobilon®-FL a une dimension de pores nominale de 0,45 μm et est recommandée pour le blotting de protéines d'un poids moléculaire supérieur à 20 kilodaltons (kDa). La membrane présente une très faible autofluorescence sur une large plage de longueurs d'onde d'excitation et d'émission. Cela en fait la solution idéale pour les applications de transfert sur membrane ("blotting") avec immunodétection par fluorescence.
Code du filtre : IPFL
Code du filtre : IPFL
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
Protein Blotting Survival Handbook: Tips and Tricks
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Could you confirm if the IPFL07810 Immobilon membrane is compatible with a specific imaging system? I need to verify if this product will work for my requirements.
1 answer-
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