ECM555
QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Synonyme(s) :
8 μm pore cell invasion assay
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About This Item
Code UNSPSC :
12352207
eCl@ss :
32161000
Nomenclature NACRES :
NA.32
Produits recommandés
Niveau de qualité
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
QCM
Technique(s)
activity assay: suitable
cell based assay: suitable
Méthode de détection
fluorometric
Conditions d'expédition
wet ice
Catégories apparentées
Description générale
QCM ECMatrix Cell Invasion Assay involves invaded cells at the bottom of the insert membrane and can be obtained by treating the membrane with cell detachment buffer. The cells are lysed and detected by the patented CyQuant GR dye. This dye is a green-fluorescent dye and binds well with cellular nucleic acids to emit a strong fluorescence. This assay kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Application
QCM ECMatrix Cell Invasion Assay, 96-well (8 μm), fluorimetric has been used in the cell invasion assay of:
- human cervical carcinoma
- osteosarcoma
- breast cancer cells
- colorectal cancer cells
Caractéristiques et avantages
- The assay does not require cell labeling, scraping, washing, or counting
- Suitable for large-scale screening and quantitative comparison of multiple samples due to homogenous fluorescence detection and 96 well insert
- Enables a quick and efficient system for the quantitative determination of several factors for cell invasion
Composants
Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.
96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
CyQuant GR Dye: (Part No. 90132) One vial - 75 μL
96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
CyQuant GR Dye: (Part No. 90132) One vial - 75 μL
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
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