ABN910

Anti-Aquaporin 4

from rabbit, purified by affinity chromatography

• Synonyme(s) : AQP-4, Mercurial-insensitive water channel, MIWC
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse, rat
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_001257487.1
• Numéro d'accès UniProt: P47863
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AQP4(361) ; mouse ... Aqp4(11829) ; rat ... Aqp4(25293)

Description

Description générale

Aquaporin-4 (UniProt: P47863; also known as AQP-4, Mercurial-insensitive water channel, MIWC) is encoded by the Aqp4 gene (Gene ID: 25293) in rat. Aquaporin-4 is a multi-pass membrane homotetrameric protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).

Spécificité

This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.

Immunogène

Epitope: C-terminus

GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.

Application

Anti-Aquaporin 4, Cat. No. ABN910, is a highly specific rabbit polyclonal antibody that targets Aquaporin 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.

Research Category
Neuroscience

Qualité

Evaluated by Immunohistochemistry (Paraffin) in mouse cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution of this antibody detected Aquaporin 4 in mouse cerebral cortex tissue.

Description de la cible

~34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.