AB5927
Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3
Chemicon®, from guinea pig
Synonyme(s) :
ASIC3, Amiloride-sensitive Cation Channel 3, TNaC1, Testis Sodium Channel 1, Dorsal Root Ganglion ASIC (DRASIC)
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50 μL
641,00 $
641,00 $
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Changer de vue
50 μL
641,00 $
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
641,00 $
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Devis pour commande en gros
Produits recommandés
Source biologique
guinea pig
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Spécificité
ASIC3 (DRASIC)
Immunogène
Epitope: 285-304 of extracellular domain of rat ASIC3
Synthetic peptide from the extracellular domain of rat ASIC3 (Catalog Number AG366). (C)TASLDPDDFDPEPSDPLGSP
Application
Detect Acid Sensitive Ion Channel 3 using this Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3 validated for use in IC, IH.
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5927
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Immunocytochemistry: 1:100
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5927
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuroinflammation & Pain
Ion Channels & Transporters
Neuroinflammation & Pain
Forme physique
Affinity purified immunoglobulin. Liquid in PBS, pH 7.4 with 1% BSA and 0.05% sodium azide.
Stockage et stabilité
Maintain at -20°C in undiluted for up to 6 months. Avoid repeated freeze/thaw cycles.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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