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Pronase® Protease, Streptomyces griseus, Nuclease-Free

Widely used broad-spectrum protease mixture

Synonyme(s) :

Nuclease-Free Protease, Streptomyces griseus, Pronase® Protease, Pronase® Protease, Streptomyces griseus

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About This Item

Numéro CAS:
9036-06-0
Numéro MDL:
MFCD00132092
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54

Forme

lyophilized

Activité spécifique

≥70,000 proteolytic units/g dry wt

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Solubilité

water: 10 mg/mL

Activité étrangère

Nuclease, none detected

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

Application

Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
  • in single-cell preparations for cardiac side population (CSP) analysis
  • in aflatoxin plasma biomarker assay
  • to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

Actions biochimiques/physiologiques

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Reconstitution

Unstable in solution; reconstitute just prior to use.

Autres remarques

Note: 1 KU = 1000 units.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Ehsan Pourkarimi et al.
eLife, 5 (2016-12-24)
The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans
Mustafa Mahfuz et al.
Journal of exposure science & environmental epidemiology, 29(5), 655-662 (2018-09-07)
Dietary exposure to aflatoxin is implicated in growth faltering of children. Despite the high burden of childhood stunting in urban Bangladesh, there are no data on long-term exposure to aflatoxin. This study aimed to explore aflatoxin exposure levels in a
Industrial Biotechnology and Commodity Products
Comprehensive Biotechnology, 3, 587-603 null
Annette Meeson et al.
Stem cell reviews and reports, 9(5), 731-739 (2013-04-27)
Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype

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