382158
Anti-Histone H3, Acetylated (1-20) Rabbit pAb
liquid, Calbiochem®
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50 μG
534,00 $
534,00 $
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50 μG
534,00 $
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
534,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
Tetrahymena sp., human
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
acetylation (Lys9,Lys14)
Informations sur le gène
human ... H3C1(8350)
Description générale
Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
Recognizes the ~17 kDa acetylated histone H3 protein.
Immunogène
Human
a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
Application
Immunoblotting (10-500 ng/ml, chemiluminescence)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Remarque sur l'analyse
Positive Control
HeLa cells
HeLa cells
Autres remarques
Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Xingang Yuan et al.
Molecular medicine reports, 14(2), 1139-1145 (2016-06-10)
The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg
Thomas Beckers et al.
International journal of cancer, 121(5), 1138-1148 (2007-04-25)
Advanced second generation inhibitors of histone deacetylases (HDAC) are currently used in clinical development. This study aimed at comparing the pharmacological properties of selected second generation HDAC inhibitors with the hydroxamate and benzamide head group, namely SAHA, LAQ824/LBH589, CI994, MS275
Haiyang Li et al.
eLife, 7 (2018-10-23)
Oomycete pathogens secrete host cell-entering effector proteins to manipulate host immunity during infection. We previously showed that PsAvh52, an early-induced RxLR effector secreted from the soybean root rot pathogen, Phytophthora sojae, could suppress plant immunity. Here, we found that PsAvh52
Atsushi Kuno et al.
Scientific reports, 8(1), 15555-15555 (2018-10-24)
Autophagy activation improves the phenotype in mdx mice, a Duchenne muscular dystrophy (DMD) model, although the underlying mechanisms are obscure. We previously found that resveratrol, a strong inducer of autophagy, ameliorates the cardiac pathology of mdx mice. Autophagy could eliminate
Takashi Hayashi et al.
Journal of biochemistry, 171(2), 201-213 (2021-11-01)
SIRT3 is an NAD+-dependent protein deacetylase localized in mitochondria. Several studies reported localization of SIRT3 in the cytoplasm or nucleus, but data of these studies were not consistent. We detected expression of mitochondrial (SIRT3mt) and cytoplasmic (SIRT3ct) Sirt3 mRNAs in
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