207000

Calcineurin Autoinhibitory Peptide

The Calcineurin Autoinhibitory Peptide controls the biological activity of Mn2+-stimulated calcineurin activity. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonyme(s) : Calcineurin Autoinhibitory Peptide, ITSFEEAKGLDRINERMPPRRDAMP
• Formule empirique (notation de Hill): C₁₂₄H₂₀₅N₃₉O₃₉S₂
• Poids moléculaire : 2930.32
• Code UNSPSC : 12352202

Propriétés

• Niveau de qualité: 100
• Pureté: ≥97% (HPLC)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Solubilité: water: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Corresponds to a C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin. Specific calcineurin inhibitor. Does not inhibit CaM kinase II, protein phosphatase I or 2A. Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated activity.

Specific calcineurin inhibitor. Corresponds to the C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin (PP2B). Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P-myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated enzyme activity. Does not inhibit CaM kinase II, protein phosphatase 1 or 2A.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Mn²⁺-stimulated calcineurin activity

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 10 µM using 32P-myosin light chain as a substrate

Conditionnement

Yes

Avertissement

Toxicity: Standard Handling (A)

Séquence

H-Ile-Thr-Ser-Phe-Glu-Glu-Ala-Lys-Gly-Leu-Asp-Arg-Ile-Asn-Glu-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-OH

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions are stable for up to 2 months at -20°C.

Autres remarques

Perrino, B.A., et al. 1995. J. Biol. Chem. 270, 340.
Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett.337, 128.
Yokoyama, N., et al. 1993. Arch. Biochem. Biophys.300, 615.
Matsui, H., et al. 1991. Biochem. Int.24, 1119.
Hashimoto, Y., et al. 1990. J. Biol. Chem.265, 1924.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable