Freeze-thaw cycles may lead to degradation of added phosphatase and protease inhibitors. It is highly recommended to prepare the cocktail just prior to application.
20-188
Tampon de lyse RIPA, 10X
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Synonyme(s) :
lysis buffer
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Sélectionner une taille de conditionnement
100 ML
153,00 $
153,00 $
Date d'expédition estimée le03 février 2025Détails
Sélectionner une taille de conditionnement
Changer de vue
100 ML
153,00 $
About This Item
Code UNSPSC :
41116010
eCl@ss :
42029053
Nomenclature NACRES :
NB.22
153,00 $
Date d'expédition estimée le03 février 2025Détails
Produits recommandés
Forme
liquid
Conditionnement
pkg of 100 mL
Fabricant/nom de marque
Upstate®
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Conditions d'expédition
ambient
Description générale
Le tampon pour analyse par radio-immunoprécipitation (tampon RIPA) facilite la lyse cellulaire et la solubilisation des protéines à partir de cultures de cellules mammifères aussi bien adhérentes qu'en suspension. De plus, ce tampon facilite l'étude des interactions protéine-protéine pertinentes en réduisant les liaisons non spécifiques entre protéines afin de maintenir un faible bruit de fond, tout en permettant à la plupart des interactions spécifiques de se produire.
Application
Forme physique
Tris-HCl 0,5 M, pH 7,4, NaCl 1,5 M, acide désoxycholique 2,5 % , NP-40 10 % et EDTA 10 mM.
Stockage et stabilité
6 mois à température ambiante.
Autres remarques
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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Can I prepare 10 ml of 1x RIPA buffer and add phosphatase and protease inhibitor then aliquot it to 1 ml Eppendorf tubes and store at -20 for use?
1 answer-
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Can this lysis extract protein from the Nucleus?
1 answer-
RIPA Buffer enables the extraction of cytoplasmic, membrane, and nuclear proteins. Certain proteins are more soluble in RIPA buffer than others.
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How long can 1x RIPA lysis buffer be stored with or without the protease?
1 answer-
This product is stable at room temperature for 6 months from date of shipment. Protease and phosphatase inhibitors should be added immediately prior to use.
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I used the 10X RIPA buffer to lyse my cultured cells. How can I do next? Will it affect the protein quantification by using BCA assay kit? Also, will it affect the SDS-PAGE and Western blot?
1 answer-
Protein samples prepared from cell culture lysates using this 1X RIPA buffer are typically compatible with downstream applications such as BCA assay, SDS-PAGE, and Western blotting. The concentrations of salts and detergents present in the 1X RIPA buffer are relatively low, minimizing interference with these techniques.
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Can we store it a 4 degree, if we want to use it after 6 months?
1 answer-
Studies have not been performed to determine if refrigerated storage will extend the shelf life of this product. The end user would have to determine suitability if used after the recommended 6 months. Note that product R0278, a 1X Ripa Buffer, is stored refrigerated and includes a warranty of 1 year from the date of shipment. Please see the link below to review this product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdfHelpful?
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Someone in lab left the 10X RIPA Buffer in room temperature for overnight. Will it affect the quality of the buffer? Could we continue to use it?
1 answer-
This product does not require refrigeration and is stable at room temperature for up to 6 months.
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Do I used distilled water to dilute to 1X?
1 answer-
The 10X RIPA Lysis Buffer should be diluted with nine parts sterile, distilled water.
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Do I have to dilute to 1X for protein isolation?
1 answer-
Yes, RIPA lysis buffer should be diluted to 1X prior to use.
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