14-420

MEK1 Protein, inactive, 50 g

Unactive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• Synonyme(s) : MEK1 Protein
• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Poids mol.: M_w 71 kDa
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable (kinase)
• Numéro d'accès NCBI: NM_002755.2
• Numéro d'accès UniProt: Q02750
• Conditions d'expédition: dry ice
• Informations sur le gène: human ... MAP2K1(5604)

Description

Description générale

Research area: CELL SIGNALING

N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1

Product Source: expressed in E.coli

MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. [1] The protein is made up of two lobes containing several conserved α-helices and β-strands. [2]

Application

Mitogen-Activated Protein Kinase Kinase 1 has been used in in vitro kinase assays:

• to elucidate the underlying mechanisms involved in the response to Rapidly Accelerated Fibrosarcoma (RAF) inhibitors and the interactions with downstream pathways. [3]
• to investigate the role of Mitogen-Activated Protein Kinase Kinase Kinase 2 (MEKK2) as a MAP3K in the Extracellular Signal-Regulated Kinase (ERK) pathway in osteoblasts and to assess its potential as a therapeutic target for treating Neurofibromatosis Type 1 (NF1).[4]
• to assess the effectiveness of the combined targeted therapy with Dabrafenib and Trametinib in treating non-small cell lung cancers (NSCLCs). [5]

Actions biochimiques/physiologiques

Protein Target: MEK1

Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. [2] One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). [1] MEK activation is closely associated with stimulating cell growth and transformation processes. [6]

Qualité

routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro

Forme physique

glutathione-agarose followed by Ni²⁺/NTA-agarose

Stockage et stabilité

6 months at -70°C

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable