05-172-AF488

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate

clone Sm1.2, from mouse, ALEXA FLUOR^™ 488

• Synonyme(s) : Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.45

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: Sm1.2, monoclonal
• Espèces réactives: chicken, rat, mouse, human, fish
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_000609
• Numéro d'accès UniProt: P05019
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IGF1(3479)

Description

Description générale

Insulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.

Spécificité

IGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).

Immunogène

Highly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).

Application

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Qualité

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.

Description de la cible

~17 kDa observed

Forme physique

Protein G Purified

Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable