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797901

Sigma-Aldrich

Ultrastable fluorescent silica nanobeads

25 nm, lyophilized

Synonyme(s) :

Fluorescent beads, Fluorescent nanoparticles, Silica nanoparticles

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2 MG
579,00 $

579,00 $

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2 MG
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About This Item

Formule linéaire :
SiO2
Poids moléculaire :
60.08
Code UNSPSC :
12352302
Nomenclature NACRES :
NA.23

579,00 $

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Devis pour commande en gros

Niveau de qualité

100

Forme

powder

Taille des particules

25 nm

Fluorescence

λem 590 nm

Absorption UV

λ: 570 nm Amax

Température de stockage

2-8°C

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Application

The use of labeling or staining agents has greatly assisted the study of complex biological interactions in biology and the fluorescent labeling of biomolecules has been demonstrated as an indispensable tool in many biological studies. By virtue of excellent fluorescence properties the silica nanobeads find application in biological labeling; bioimaging; biosensing etc.[1][2] Ultrastable particles exhibit a very high resistance to photobleaching and can be used in single-particle imaging as well as in super resolution techniques such as stimulated emission depletion (STED) microscopy.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
MKCW6820
MKCS7648
MKCP6479
MKCN0035
MKCK9897

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Luminescent nanomaterials for biological labelling
F. Wang, W. B. Tan, Y. Zhang, et al.
Nanotechnology, 17(1), R1-R13 (2006)
Geometrical confinement of quantum dots in porous nanobeads with ultraefficient fluorescence for cell-specific targeting and bioimaging
P-J Chen, S-H Hu, W-T Hung, S-Y Chen, and D-M Liu,
Journal of Materials Chemistry, 22(19), 9568-9575 (2012)

Articles

Analysis of Polar Compounds with Ion Pair Reagents

RAFT polymerization controls chain growth, yields defined polymers without cytotoxic heavy metals like ATRP.

Micro- and Nanofabricated Biomaterials

Biomaterials science integrates smart materials into biological research, requiring a deep understanding of biological systems.

Questions

1–3 of 3 Questions  

  1. I applied probe sonication on ice for 8 minutes at 30% amplitude. However, the DLS size measurement results showed the particle size of is around 300–800 nm, which differs from the product description (25 nm). How?

    1 answer

    1. The 25 nm nanobeads may exhibit high surface energy, making them prone to aggregation in solvent, which could explain the observed particle size of 300–800 nm in DLS measurements. It's also important to note that the 30% amplitude setting does not provide a clear indication of the actual power being applied during sonication. For better results, it is generally recommended to use a protocol of 1 second on with 3 seconds off for a total of 15 minutes under 400 Watts. Additionally, checking TEM images for the particle size, if available, may provide more accurate information on the actual size of the nanobeads.

      Helpful?

  2. Are these PFA-fixable?

    1 answer

    1. A specific PFA Fixation study is not performed on this material. The literature around this topic seems to suggest this is a possible use for this product and more details can be found below.

      Chiou AE, Hinckley JA, Khaitan R, Varsano N, Wang J, Malarkey HF 5th, Hernandez CJ, Williams RM, Estroff LA, Weiner S, Addadi L, Wiesner UB, Fischbach C. Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments. Small. 2021 Apr;17(15):e2001432. doi: 10.1002/smll.202001432. Epub 2020 May 28. PMID: 32462807; PMCID: PMC7704907.

      Further application information can be found on the website through the link below.

      https://www.sigmaaldrich.com/technical-documents/technical-article/materials-science-and-engineering/biosensors-and-imaging/fluorescent-silica-nanobeads

      Helpful?

  3. what is dispersibility of this beads in water.

    1 answer

    1. The nanopowder may be re-dispersed by adding water directly to the storage vial. It is recommended to add 1 mL of water per each 1 mg of nanoparticles to be re-dispersed, and probe sonicating on ice for 8 minutes at 30% amplitude or until the powder has completely re-dissolved.

      Helpful?

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