Biochimica et biophysica acta, 1409(1), 50-57 (1998-11-07)
Site-directed mutagenesis was used to produce a Synechocystis mutant containing a histidine tag at the C terminus of the CP 47 protein of Photosystem II. This mutant cell line, designated HT-3, exhibited slightly above normal rates of oxygen evolution and
The mechanism of peroxidative N-dealkylation of alkylamines proceeds via one-electron oxidation to the iminium cation which reacts with water to give the N-hydroxymethyl derivative which decomposes to formaldehyde and the N-demethylated product. This reaction is normally inhibited by glutathione by
Archives of biochemistry and biophysics, 228(2), 439-442 (1984-02-01)
It has been demonstrated previously (P.C. Andrews and N.I. Krinsky (1981) J. Biol. Chem. 256, 4211-4218) that human leukocyte myeloperoxidase, an alpha 2 beta 2 enzyme, can be cleaved by mild reduction and alkylation to an alpha 1 beta 1
Journal of clinical microbiology, 29(8), 1596-1603 (1991-08-01)
Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized.
The intra-axonal transport of horseradish peroxidase (HRP) has been used to trace the nodose ganglion and brain stem projections of a physiologically distinct nerve - the aortic depressor nerve - following electrophysiological identification. Tetramethyl benzidine (TMB) has been used as
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