Stock solutions retain activity after 5 freeze-thaw cycles when stored at –20°C. Studies at –80°C have not been conducted. For additional solution stability information. see the product datasheet for the research grade product, P8375.
A link to this document is provided below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/160/616/p8375dat-ms.pdf
SRE0082
Peroxidase from horseradish
Synonym(s):
Peroxidase from horseradish, Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
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5000 UNITS
$196.00
30000 UNITS
$856.00
300000 UNITS
$7,520.00
1000 KU
$15,810.00
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$63,220.00
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5000 UNITS
$196.00
30000 UNITS
$856.00
300000 UNITS
$7,520.00
1000 KU
$15,810.00
5000 KU
$63,220.00
About This Item
Recommended Products
description
For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US.
Quality Level
specific activity
≥250 units/mg solid (using pyrogallol)
shipped in
wet ice
storage temp.
2-8°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
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General description
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
Application
Peroxidase from horseradish has been used in western blotting.[1]
Unit Definition
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Analysis Note
The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
Other Notes
View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.
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What is the stability of the activity after reconstituted into PBS if stored at -20 or -80C?
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