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P4682

Sigma-Aldrich

Phenol solution

BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, for molecular biology

Synonym(s):

nucleic acid extraction

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About This Item

Empirical Formula (Hill Notation):
C6H6O
CAS Number:
108-95-2
Molecular Weight:
94.11
Beilstein:
6428463
MDL number:
MFCD00002143
UNSPSC Code:
12352104
PubChem Substance ID:
57654523
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

liquid

technique(s)

RNA purification: suitable

pH

4.1-4.5 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

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General description

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

  • for the extraction of RNA by standard phenol-chloroform protocols
  • in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves
  • as a component of lysis-denaturation buffer for RNA isolation

Biochem/physiol Actions

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.

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Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Target Organs

Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli
Levanon SS, et al.
Metabolic engineering, 7(5-6), 364-374 (2005)
mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils
Staudinger BJ, et al.
The Journal of Clinical Investigation, 110(8), 1151-1163 (2002)
PHENOLS
Smith PWG and Tatchell AR
Aromatic Chemistry, 79-104 (1969)
Sagit Shalel-Levanon et al.
Biotechnology and bioengineering, 92(2), 147-159 (2005-07-01)
Escherichia coli has several elaborate sensing mechanisms for response to the availability of oxygen and the presence of other electron acceptors. The adaptive responses are coordinated by a group of global regulators, which include the one-component Fnr protein, and the
Louise Jones et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(20), 11783-11788 (2003-09-18)
Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls
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