MAK016
Glycogen Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
Glycogen is a branched polymer of glucose that serves as the primary short-term energy storage molecule in animals. Glycogen is primarily synthesized in liver and muscle tissue where it can constitute up to 10% of the weight of liver and 1-2% of the weight of muscle tissue. While muscle glycogen is generally utilized locally, liver glycogen serves as an important buffer to regulate blood glucose levels. Glycogen metabolism is dysregulated in diabetes and the glycogen storage diseases due to inborn errors of metabolism.
Application
Glycogen Assay Kit has been used in glycogen quantification. It has also been used to determine the glycogen content in liver, vastus lateralis and muscle homogenates.
Suitability
Suitable for determining glycogen concentration in various tissues such as liver etc. and cell culture (adherent or suspension cells).
Principle
Glycogen concentration is determined by a coupled enzyme assay, which produces a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the glycogen present.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content.
Nieman D C, et al.
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