E6511

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

BioReagent, suitable for cell culture, 98.5-102.0%

• Synonym(s): EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate
• Linear Formula: (NaOOCCH₂)₂NCH₂CH₂N(CH₂COONa)₂ · 2H₂O
• CAS Number: 10378-23-1
• Molecular Weight: 416.20
• Beilstein: 3861753
• EC Number: 200-573-9
• MDL number: MFCD00150026
• UNSPSC Code: 12352204
• PubChem Substance ID: 24894629
• NACRES: NA.75

Properties

• product line: BioReagent
• Quality Level: 200
• Assay: 98.5-102.0%
• form: powder
• technique(s): cell culture | mammalian: suitable
• pH range: 11.0-11.7
• solubility: H₂O: 160 mg/mL
• shipped in: ambient
• storage temp.: room temp
• SMILES string: O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4
• InChI key: KSYNLCYTMRMCGG-UHFFFAOYSA-J

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is a chelator of metal ions.[1] It is a substituted diamine, which has antibacterial activity. EDTA removes the undesirable effects of ferric, cupric and manganic ions in bleaching. It prevents cellular division, chlorophyll synthesis and algal biomass production.[2] EDTA is an inhibitor of metalloprotease.[1] It has anticoagulant property.[3]

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:

• as a chelating agent to isolate human endometrial stem cell/stromal cells (hEnSCs) from menstrual blood[4]
• as a chelator in animal and testicular cell isolation[5]
• as a chelator for the culture of neural stem cells[6]

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H332,H318,H373
• Precautionary Statements: P260 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves