Skip to Content
MilliporeSigma
All Photos(4)

Key Documents

EDS

Sigma-Aldrich

Ethylenediaminetetraacetic acid

Ethylenediaminetetraacetic acid

BioUltra, anhydrous, ≥99% (titration)

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

Sign Into View Organizational & Contract Pricing

Select a Size

100 G
$48.70
500 G
$126.00
1 KG
$230.00

$48.70


In StockDetails


Request a Bulk Order

Select a Size

Change View
100 G
$48.70
500 G
$126.00
1 KG
$230.00

About This Item

Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
EC Number:
MDL number:
UNSPSC Code:
12352116
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.77

$48.70


In StockDetails


Request a Bulk Order

grade

anhydrous

Quality Level

product line

BioUltra

Assay

≥99% (titration)

form

powder

reaction suitability

reagent type: chelator

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

water: soluble 0.4 g/L at 20 °C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Ethylenediaminetetraacetic acid (EDTA) is a substituted diamine and a versatile compound in household with industrial applications. EDTA chelates metal divalent cations like calcium, magnesium, zinc, copper, manganese by forming metal-EDTA complexes. It finds its application in cleaning products and detergent formulations. In paper and pulp industries, EDTA reduces the adverse effect of metal ions on bleaching. It functions as an antibacterial agent by removing calcium and magnesium divalent cations from the outer membrane and causes loss of lipopolysaccharide from the membrane, making the bacteria susceptible to bactericidal agents. Free EDTA has adverse effect on reproduction and development in mammals.[1] EDTA can chelate ions from deposits that contribute to atherosclerosis, cancers and heart diseases. It can help to overcome cell membrane injury by reducing radical reactions and oxidation processes. It binds to calcium and reduces the risk of hypercalcinemia in cancer patients. Transmembrane ammonium EDTA gradient method aids in drug delivery, increasing drug retention and efficacy of therapeutics and lowers the cytotoxicity levels.[2]

Application

EDTA has been used:
  • as a medium supplement in embryo culture using synthetic oviduct fluid culture 1 (SOFC1) medium[3]
  • in incubation of EpCAM+ cells with fluorescein isothiocyanate (FITC) conjugated antibody for cell sorting analysis[4]
  • in pre-treatment of single cell suspension of dendritic cells from spleen for cell sorting with magnetic beads[5]

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Repetitive injections of dendritic cells matured with tumor necrosis factor alpha induce antigen-specific protection of mice from autoimmunity
Menges M, et al.
The Journal of Experimental Medicine, 195(1), 15-22 (2002)
The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
Song Y, et al.
International journal of nanomedicine, 9, 3611-3621 (2014)
Effect of the absence or presence of various protein supplements on further development of bovine oocytes during in vitro maturation
Ali A and Sirard M
Biology of Reproduction, 66(4), 901-905 (2002)
Caio A C G Brunharo et al.
Journal of agricultural and food chemistry, 67(31), 8431-8440 (2019-05-09)
Glufosinate-resistant Lolium perenne L. spp. multiflorum biotypes from Oregon exhibited resistance levels up to 2.8-fold the field rate. One resistant biotype (MG) had an amino acid substitution in glutamine synthetase 2 (GS2), whereas the other (OR) exhibited the wild-type genotype.
The phenotypes of pluripotent human hepatic progenitors
Schmelzer E, et al.
Stem Cells, 24(8), 1852-1858 (2006)

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service