11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Synonym(s): buffer
• UNSPSC Code: 41105600

Properties

• form: solution
• Quality Level: 100
• packaging: pkg of 3 × 1 mL
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

General description

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Application

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).[1][2]

Physical form

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Other Notes

For general laboratory use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash