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420127

Sigma-Aldrich

Jasplakinolide

from Jaspis johnstoni, ≥90% (HPLC), liquid, Actin polymerization promotor, Calbiochem

Synonym(s):

InSolution Jasplakinolide, Jaspis johnstoni

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About This Item

Empirical Formula (Hill Notation):
C36H45BrN4O6
Molecular Weight:
709.67
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Jasplakinolide, Jaspis johnstoni, InSolution, ≥90%, 1 mM in DMSO, mammablian cell mitosis inhibitor, potent inducer of actin polymerization and stabilization

Quality Level

100

Assay

≥90% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

shipped in

wet ice

storage temp.

−20°C

General description

A cell-permeable F-actin probe. This macrocyclic cyclodepsipeptide, isolated from the marine sponge Jaspis johnstoni, is a potent inducer of actin polymerization and stabilization in vitro. It competes with Phalloidin (Cat. No. 516640) for actin binding (Kd = 15 nM) and enhances apoptosis induced by interleukin 2 (IL-2) deprivation. Also displays fungicidal, insecticidal, and anticancer properties.

Biochem/physiol Actions

Cell permeable: no
Kd = 15 nM for actin binding
Primary Target
F-actin
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Physical form

A 1 mM (50 µg/71 µl) solution of Jasplakinolide, Jaspis johnstoni (Cat. No. 420107) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Rosado, J.A., et al. 2000. J. Biol. Chem.275, 7527.
Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.
Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.
Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.
Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Flash Point(C)

87 °C - closed cup - (Dimethylsulfoxide)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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