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07-875

Sigma-Aldrich

Anti-phospho-PKCδ (Ser664) Antibody

Upstate®, from rabbit

Synonym(s):

PRKCD, PKC delta, PKCd, Protein Kinase C, delta

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_006254.3
NM_212539.1

UniProt accession no.

Q05655

shipped in

dry ice

target post-translational modification

phosphorylation (pSer664)

Gene Information

human ... PRKCD(5580)

Specificity

Predicted to cross-react more weakly with mouse based on sequence homology, but have not been tested.
Recognizes phospho-PKCδ (Ser664), weaker with with mouse and rat PKCδ (Ser662) and PKCα (Ser657) and PKCβII (Ser662).

Immunogen

Peptide corresponding to amino acid region encompassing the human phospho-PKCδ (Ser664).

Application

Detect phospho-PKCδ (Ser664) using this Anti-phospho-PKCδ (Ser664) Antibody validated for use in WB.
Immunoblot Analysis: A 1:1,000 dilution of this lot detected phospho-PKCδ (Ser664) in RIPA lysate of PMA-treated K562 cells.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by immunoblot.

Target description

78 kDa

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified

Storage and Stability

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Signaling networks downstream of receptor tyrosine kinases are among the most extensively studied biological networks, but new approaches are needed to elucidate causal relationships between network components and understand how such relationships are influenced by biological context and disease. Here, we investigate

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