D4522

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

• Synonym(s): Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
• CAS Number: 91080-16-9
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• EC Number: 293-507-3
• NACRES: NA.52
• MDL number: MFCD00151698
• Biological source: bovine pancreas
• Form: lyophilized powder
• Solubility: water: slightly soluble
• Storage temp.: −20°C

Properties

• biological source: bovine pancreas
• Quality Segment: 200
• grade: Molecular Biology
• type: Type XV
• form: lyophilized powder
• solubility: water: slightly soluble
• storage temp.: −20°C

Description

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

One mg DNA is approx. 20 A₂₆₀ units.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves