For cellulase (C2730) the optimal pH range is 4.5 - 6.0, and the optimal temperature range is 50 - 60°C, as shown in the graphic below.
C2730
Cellulase from Trichoderma reesei
Greener Alternative
aqueous solution, ≥700 units/g
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
eCl@ss:
32160410
Specific activity:
≥700 units/g
Biological source:
fungus (Trichoderma reesei)
Pricing and availability is not currently available.
biological source
fungus (Trichoderma reesei)
Quality Segment
form
aqueous solution
specific activity
≥700 units/g
mol wt
68 kDa
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
density
1.10-1.30 g/mL
greener alternative category
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Cellulase from Trichoderma reesei is produced by submerged fermentation of a selected strain of the fungus Trichoderma reesei and catalyzes the breakdown of cellulose into glucose, cellobiose, and higher glucose polymers. It corresponds to a molecular mass of 68 kDa with optimum pH and temperature of 6 and 52° C, respectively.
Cellulase from Trichoderma reesei is produced by submerged fermentation of a selected strain of the fungus Trichoderma reesei and catalyzes the breakdown of cellulose into glucose, cellobiose, and higher glucose polymers. It corresponds to a molecular mass of 68 kDa with optimum pH and temperature of 6 and 52° C, respectively.
Application
Cellulase from Trichoderma reesei has been used:
- for aqueous extraction of oil from corn germ
- in the enzymatic hydrolysis of Sweet Sorghum Bagasse (SSB)
- in the enzymatic digestion of paper grids
Biochem/physiol Actions
Cellulase complexes with β-glucosidase for degrading cellobiose to glucose polymer. The lignin content in straw and furfural residues hampers cellulase production.
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This Item | |||
|---|---|---|---|
| description aqueous solution, ≥700 units/g | description powder, ≥5,000 units/g solid | description BioReagent, suitable for plant cell culture, 3-10 units/mg solid | description aqueous solution |
| specific activity ≥700 units/g | specific activity ≥5,000 units/g solid | specific activity 3-10 units/mg solid | specific activity ≥1000 units/g |
| biological source fungus (Trichoderma reesei) | biological source - | biological source fungus (Trichoderma sp.) | biological source - |
| form aqueous solution | form powder | form powder | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 68 kDa | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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What is the working pH and temperature, please?
1 answer -
Using Cellulases (C2730) and noticed a peak around 20–22 minutes in HPLC with a carbohydrate column. Could this product contain residual sugars (e.g. glucose, oligosaccharides) from the fermentation medium or added stabilizers like sugar alcohols?
1 answer-
This product contains sorbitol, which is a sugar alcohol.
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What is the molecular weight of the enzyme, or the average molecular weight of the enzymes in the cocktail?
1 answer-
As listed in the 'PROPERTIES' section, the molecular weight of this enzyme is approximately 68 kDa.
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How many types of enzyne contian in Product C2730, cellulase?
1 answer-
The types of enzymes contained in this product are proprietary.
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What does the aqueous solution in which the cellulases are stored contain?
1 answer-
While the specific formulation of this material is considered proprietary, this aqueous solution includes sodium chloride and potassium sorbate.
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Is this product food grade and can be use for cellulosic part of a food material
1 answer-
This product is not a food grade Cellulase from Trichoderma reesei.
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What is the best buffer (including molarity & pH) for C2730 (cellulase)?
1 answer-
The best buffer needed would depend on the application.
Please see the link below to review the enzyme assay performed on the starting material for this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/352/413/c8546enz.pdfIt may also be helpful to review the following literature:
https://www.sciencedirect.com/science/article/pii/S2452014417300638#s0010
https://www.sciencedirect.com/science/article/pii/S1096717608000396?via%3Dihub
https://www.jstage.jst.go.jp/article/jpi/60/6/60_322/_pdfHelpful?
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Is any nuclease activity tested for the Cellulase (C2730) ?
1 answer-
Cellulase (C2730) has not been tested for nuclease activity. Currently, there is not a cellulase product that is tested for nuclease, protease, DNAse, or RNAse.
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What are the optimal conditions for Product C2730, Cellulase?
1 answer-
For cellulase (C2730) the optimal pH range is 4.5 - 6.0, and the optimal temperature range is 50 - 60°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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