For testing purposes, this product is prepared in cold deionized water. While PBS has not been tested as a solvent, the enzyme is expected to be soluble. Please see the link below to review the assay protocol for cellulase and hemicellulase:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-cellulase
C1794
Cellulase from Trichoderma sp.
BioReagent, suitable for plant cell culture, 3-10 units/mg solid
Greener Alternative
Synonym(s):
β-1,4-glucanase
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About This Item
CAS Number:
UNSPSC Code:
10171502
eCl@ss:
32160410
EC Number:
232-734-4
NACRES:
NA.72
MDL number:
EC Number:
Specific activity:
3-10 units/mg solid
Biological source:
fungus (Trichoderma sp.)
Pricing and availability is not currently available.
biological source
fungus (Trichoderma sp.)
Quality Segment
product line
BioReagent
form
powder
specific activity
3-10 units/mg solid
contains
maltodextrin
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
cell culture | plant: suitable
application(s)
agriculture
greener alternative category
, Enabling
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
Cellulase (β-1,4-glucanase) is a natural enzyme produced by the filamentous fungi Trichoderma viride which promotes the endohydrolysis of (1→4)-beta-D-glucosidic linkages in cellulose and lichenin. Cellulases are important for processing potential commercial products such as cellulosic ethanol.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
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This Item | |||
|---|---|---|---|
| description BioReagent, suitable for plant cell culture, 3-10 units/mg solid | description suitable for plant cell culture, lyophilized powder | description powder, ≥5,000 units/g solid | description powder, deep brown, ≥4 U/mg |
| biological source fungus (Trichoderma sp.) | biological source - | biological source - | biological source fungus (Agaricus bisporus) |
| specific activity 3-10 units/mg solid | specific activity ≥0.3 unit/mg solid | specific activity ≥5,000 units/g solid | specific activity ≥4 U/mg |
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) - | technique(s) - |
| form powder | form lyophilized powder | form powder | form powder |
| application(s) agriculture | application(s) agriculture | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Hi, I was wondering how to prepare the enzyme solution? Can I just dilute it on PBS? Thanks
1 answer-
Helpful?
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Hello, what is the Uniprot number or Gene ID for C1794-5KU? Thank you
1 answer-
The Gene ID for Cellulase from Trichoderma sp. (AKA Gliocladium sp.) is GH45. Please see information at the links below:
Helpful?
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