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YTA 3.1.2

89040603, Reactivity Anti-L3T4 epitope B, mouse CD4 )

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

ImmunoDon

DA rat spleen

Myeloma

Y3Ag1.2.3

Reactivity

Anti-L3T4 epitope B, mouse CD4

technique(s)

cell culture | mammalian: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Not specified

Cell Line Description

The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.

Immunogen

CBA mouse spleen cells, thymocytes

Application

Not specified

Culture Medium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Subculture Routine

Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37 °C. Saturation density 9 x 105/ml

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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