Undifferentiated SH-SY5Y cells typically resemble immature catecholaminergic neurons. These cells are characterized by markers indicative of proliferation, such as proliferating cell nuclear antigen (PCNA), as well as by immature neuronal markers such as nestin. Evidence shows that through continuous subculture (or passaging), SH-SY5Y cells start to lose their neuronal characteristics and the potential to generate neurites. The consensus is that cells should be maintained below passage (P) 20. It is therefore important that you manage your stocks of the cell line appropriately. Typically, SH-SY5Y cells are supplied at around P12. It is recommended to subculture the cells at a ratio of 1:10 or higher, seeding at a maximum of 1E4 cells/cm2 and that cryopreserved working stocks of the cells be prepared within two passages of receipt (i.e. around 30 vials at P14). Using this strategy, splitting at a split ratio of 1:10, each vial of working stock would be capable of being expanded 1E6 (up to 1 million times) before the arbitrary P20 limit. Seeding at higher split ratios (up to 1:100) and lower seeding densities (as low as 1,000 cells/cm2) can further maximize the use of these cells.
SH-SY5Y Cell Line human
94030304, human nerve, Neuroblast
Synonym(s):
SHSY5Y Cells, SK-SH-SY5Y Cells, SY5Y Cells
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About This Item
UNSPSC Code:
41106514
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Product Name
SH-SY5Y Cell Line human, 94030304, Neuroblast from neural tissue.
biological source
human nerve
growth mode
Adherent
karyotype
Not specified
morphology
Neuroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human neuroblastoma
Cell Line Description
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
Application
SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:
SH-SY5Y cell line human has been used to:
- study the iron-mediated toxicity of amyloid β peptide[1]
- check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells[2]
- study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells[3]
Other Notes
- DNA is stored at -70 °C
- RNA is stored at -70 °C
- Cell line is stored at -196 °C
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DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
Culture Medium
Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% Trypsin or Trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
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Hello, I would like to ask about this SH-SY5Y line (94030304). I am using them for testing proliferative capacity (pasage 14-16). If they are not differentiated, are they acting as neuronlike or more cancerous like? Thank you.
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