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AB7821

Sigma-Aldrich

Anti-Collagen Type VI Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL6A1(1291)

Specificity

Human collagen type I <0.5%

Human collagen type II <0.5%

Human collagen type III <0.5%

Human collagen type IV <0.5%

Human collagen type V 5%

Human collagen type VI 100%

Human fibronectin <0.5%

Human albumin <0.5%

Human immunoglobulin <0.5%

Immunogen

Human placental collagen type VI

Application

Anti-Collagen Type VI Antibody is an antibody against Collagen Type VI for use in ELISA, IH.
Immunohistochemistry: 1:10-1:40 dilution of reconstitute for indirect immunofluorescent staining of frozen tissue sections.

ELISA: 1:10,000 dilution of reconstitute for ELISA on human collagen type VI.

Optimal working dilutions must be determined by the end user.

Physical form

Pooled antisera was cross-absorbed against immobilized human serum proteins and collagen types IV and V. The IgG fraction was then precipitated with ammonium sulfate and subsequently dialyzed. Lyophilized from 500μL of 10mM Na-phosphate, 150mM NaCl, pH 7.5 with 0.1% mannitol.

Storage and Stability

Maintain lyophilized at -20°C for up to 12 months. Reconstitute with 500uL of distilled water or saline. Store reconstitute between 2 and 8°C for up to two months. Remove any insoluble material by microcentrifugation before use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tyler Novak et al.
Advanced functional materials, 26(16), 2617-2628 (2016-06-28)
Biological tissues and biomaterials are often defined by unique spatial gradients in physical properties that impart specialized function over hierarchical scales. The structure and organization of these materials forms continuous transitional gradients and discrete local microenvironments between adjacent (or within)
Valentina Emmanuele et al.
Human molecular genetics, 24(3), 714-726 (2014-10-03)
A member of the four-and-a-half-LIM (FHL) domain protein family, FHL1, is highly expressed in human adult skeletal and cardiac muscle. Mutations in FHL1 have been associated with diverse X-linked muscle diseases: scapuloperoneal (SP) myopathy, reducing body myopathy, X-linked myopathy with
Xin Xu et al.
Developmental biology, 418(2), 242-247 (2016-09-01)
The pericellular matrix (PCM) is a component of the extracellular matrix that is found immediately surrounding individual chondrocytes in developing and adult cartilage, and is rich in the proteoglycan perlecan. Mutations in perlecan are the basis of several developmental disorders
Three novel collagen VI chains with high homology to the alpha3 chain.
Gara, SK; Grumati, P; Urciuolo, A; Bonaldo, P; Kobbe, B; Koch, M; Paulsson, M; Wagener, R
The Journal of Biological Chemistry null
S San Martin et al.
Journal of tissue engineering and regenerative medicine, 7(1), 10-19 (2011-11-05)
Several studies have developed efficient oral mucosa constructs using different types of scaffold. However, the changes in the morphology and gene and protein expression profile that could occur in these artificial constructs remain unknown. This study compared the histology and

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