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MABD396

Sigma-Aldrich

Anti-Sortilin Antibody, clone 4E3.1

clone 4E3.1, from mouse

Synonym(s):

Sortilin, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.75

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4E3.1, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

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This Item
MABN1792AB9712AMAB91428
clone

4E3.1, monoclonal

clone

F11, monoclonal

clone

polyclonal

clone

CL6528, monoclonal

species reactivity

human, mouse

species reactivity

mouse, human

species reactivity

human, mouse, rat

species reactivity

mouse, human

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

-

Gene Information

human ... SORT1(6272)

Gene Information

human ... SORT1(6272)

Gene Information

human ... SORT1(6272)

Gene Information

human ... SORT1(6272)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

General description

Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the a family of sorting receptors characterized by the Vps10p domain that creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. The family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reproted to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit reduced PCSK9 plasma concentration and altered PCSK9 subcellular localization in hepatocytes. Sortilin is also found to colocalize with APP in neurites and facilitates APP processing toward the production of sAPPα, possibly by optimizing APP as a substrate for α-secretase cleavage. Sortilin is initially produced with a signal peptide (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region (a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Similar to the other two family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.

Specificity

Expected to react with both spliced isoforms reported by UniProt (Q99523).

Immunogen

Epitope: C-terminal region.
GST-tagged recombinant human Sortilin C-terminal fragment.

Application

Anti-Sortilin Antibody, clone 4E3.1 is an antibody against Sortilin for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected Sortilin in human cerebral cortex, colon, and kidney tissue sections.
Research Category
Stem Cell Research
Research Sub Category
Vesicular Trafficking

Quality

Evaluated by Western Blotting in E13-14 embryonic mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sortilin in 10 µg of E13-14 embryonic mouse brain tissue lysate.

Target description

~92 kDa observed. Target band size appears larger than the calculated molecular weights of 83.86 kDa (isoform 1) and 69.17 kDa (isoform 2) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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