Supel^™-Select Polymeric SPE

HLB and Ion-Exchange phases for a wide range of applications and pH conditions

Supel™-Select SPE phases are ideal for the solid phase extraction of a broad range of compounds from aqueous samples. While reversed-phase interactions dominate retention on the Supel™-Select HLB, and the retention mechanisms of the Supel™-Select SAX and SCX are predominately based on ion-exchange, the hydrophilic modifications of the styrene-based polymer backbone allow for retention and recovery of more polar compounds.

Table 1.

HLB Phase Chemistry:	Hydrophilic modified styrene polymer
SAX Phase Chemistry:	Quaternary amine functionalized hydrophilic modified styrene polymer
SCX Phase Chemistry:	Sulfonic acid functionalized hydrophilic modified styrene polymer
pH Compatibility:	0-14
Particle Size:	50-70 µM
MS Suitability:	Yes
Surface Area:	160-420 m2/g
Pore Volume:	0.8-1.2 mL/g
Pore Size:	80-200 Å

Table 2.

Feature	Benefit
Hydrophilic modified styrene resin	Extract and recover a broad range of analytes (polar to non-polar, acidic to basic) from aqueous samples
Method simplicity	Save time, money, and frustration during method development
Greater capacity	Allows for smaller bed weights = smaller elution volumes = time savings in sample processing
Resistant to overdrying	More robust methodology and better reproducibility
Low UV and MS extractables	Lower background for greater sensitivity
Stringent production & QC	Greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

High and Reproducible Recoveries

The hydrophilic, lipophilic balanced Supel™-Select HLB SPE allows users to extract a broad range of compounds using a single sorbent and generic methodology. Analyte recovery was high across all the compounds tested, and results were highly reproducible across three production lots.

Minimal Extractables

Supel™-Select HLB SPE phases are quality controlled for both LC-UV and LC-MS/MS extractables. As assays require greater sensitivity, analytical “cleanliness” of both SPE phases and associated hardware becomes more critical.
In this simple test, acetonitrile was passed through both Supel™-Select HLB SPE tubes and the leading competitor’s tubes followed by LC-UV analysis.

Supel™-Select HLB SPE imparted less than 25% of UV extractables than the leading competitor.

Figure 1.