Pular para o conteúdo
Merck

Methods to study monocyte migration induced by HIV-infected cells.

Methods in molecular biology (Clifton, N.J.) (2008-11-21)
Vasudev R Rao, Eliseo A Eugenin, Joan W Berman, Vinayaka R Prasad
RESUMO

HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep, affinity isolated antibody, buffered aqueous solution