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Merck

Biothiol-triggered, self-disassembled silica nanobeads for intracellular drug delivery.

Acta biomaterialia (2015-05-20)
Xin-Chun Huang, Li-Bang Wu, Jen-Fang Hsu, Shinsuke Shigeto, Hsin-Yun Hsu
RESUMO

Silica-based nanomaterials have demonstrated great potential in biomedical applications due to their chemical inertness. However, the degradability and endosomal trapping issues remain as rate-limiting barriers during their innovation. In this study, we provide a simple yet novel sol-gel approach to construct the redox-responsive silica nanobeads (ReSiNs), which could be rapidly disassembled upon redox gradient for intracellular drug delivery. The disulfide-linked scaffold of the nanobead was synthesized by employing the dithiobis-(succinimidyl propionate) to bridge (3-aminopropyl)-trimethoxysilane. Such silica matrix could be efficiently disrupted in response to intracellular glutathione, resulting in drug release and collapse of entire nanocarrier. Moreover, the ReSiNs exhibited insignificant cytotoxicity before and after the degradation. These results indicated the potential of using ReSiNs as a novel silica-based, biothiol-degradable nanoplatform for future drug delivery.

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Sigma-Aldrich
Ácido clorídrico, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Ácido clorídrico, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
(3-Aminopropyl)trimethoxysilane, 97%
Supelco
Ácido clorídrico, volumetric, 0.1 M HCl (0.1N), endotoxin free
Sigma-Aldrich
(3-Mercaptopropyl)trimethoxysilane, 95%
Sigma-Aldrich
Hidróxido de potássio, anhydrous, ≥99.95% trace metals basis
Sigma-Aldrich
Ácido clorídrico, ~6 M in H2O, for amino acid analysis
Sigma-Aldrich
Álcool etílico, puro, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
Cloreto de hidrogênio, 3 M in cyclopentyl methyl ether (CPME)
Sigma-Aldrich
Ácido clorídrico, 32 wt. % in H2O, FCC
Sigma-Aldrich
3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester), powder
Sigma-Aldrich
Álcool etílico, puro, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
5-Carboxy-tetramethylrhodamine N-succinimidyl ester, BioReagent, suitable for fluorescence
Sigma-Aldrich
Álcool etílico, puro, 190 proof, ACS reagent, meets USP testing specifications, Excise Tax-free, Permit for use required
Sigma-Aldrich
Álcool etílico, puro, 160 proof, Excise Tax-free, Permit for use required