[Determination of acetylcholine by inhibition of the specific binding of tritiated cis-methyldioxolane: application to the study of the effects of acetylcholine accumulation in a murine neuroblastoma culture].

The radioreceptor assay for acetylcholine (ACh) is based on the ability of the ACh to compete with the specific binding of [3H] cis-methyldioxolane to muscarinic receptors of rat cerebral cortex membranes. The technique described was used to measure ACh levels in NS 20 cells treated with an organophosphorus compound. The down regulation of muscarinic acetylcholine receptors of neuroblastoma cells observed is probably related to ACh accumulation.