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The molecular cloning of 8-epicedrol synthase from Artemisia annua.

Archives of biochemistry and biophysics (1999-09-16)
L Hua, S P Matsuda
RESUMO

A cDNA library was prepared from Artemisia annua, and a 129-bp fragment was amplified from this library using primers corresponding to sequences conserved in known dicot sesquiterpene synthases. A 1641-bp open reading frame that encoded a predicted protein 35-38% identical to dicot sesquiterpene synthases was cloned using this fragment as a hybridization probe. The gene product expressed in Escherichia coli cyclized farnesyl diphosphate to a 96:4 mixture of (-)8-epicedrol and cedrol. Neither cedrol epimer was detected by GC-MS in an A. annua extract prepared from the same specimen as the cDNA.

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Sigma-Aldrich
(+)-Cedrol, ≥99.0% (sum of enantiomers, GC)