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Merck

A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria.

Nature methods (2019-03-13)
Jonathan M Stokes, Arnaud Gutierrez, Allison J Lopatkin, Ian W Andrews, Shawn French, Ivan Matic, Eric D Brown, James J Collins
RESUMO

Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity-an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive staining to report on the killing of antibiotic-tolerant bacteria. This method can be used with diverse bacterial species and applied to genome-scale investigations to identify therapeutic targets against tolerant pathogens.