- Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes.
Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes.
The Journal of biological chemistry (1991-10-05)
N A Woychik, W S Lane, R A Young
PMID1918023
RESUMO
The Saccharomyces cerevisiae RNA polymerase II subunit gene RPB9 was isolated and sequenced. RPB9 is a single copy gene on chromosome VII. The RPB9 sequence predicts a protein of 122 amino acids with a molecular mass of 14,200 Da. The yeast RPB9 subunit is similar in size and sequence to a protein encoded by DNA adjacent to the suppressor of the Hairy Wing gene in Drosophila melanogaster. Deletion of the RPB9 gene produced cells that were heat- and cold-sensitive. The RPB9 subunit, like the previously described RNA polymerase II subunit RPB4, is not essential for synthesis of mRNA, but is required for normal cell growth over a wide temperature range.