1X PBST for Western Blotting

Phosphate-buffered saline (PBS) is an isotonic solution that is used in many biological research applications. To make 1 L of 1X phosphate-buffered saline with Tween^® detergent (PBST), add 100 mL of 10X PBS and 1 mL of Tween^® detergent to 900 mL of water. This PBST recipe contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 1.8 mM KH₂PO₄.

This PBST recipe calculator enables the accurate preparation of wash buffer for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed to make this common buffer used for Western blotting and other immunoassays.

1X PBST Solution concentrations:

• NaCl: 137 mM
• KCl: 2.7 mM
• Na₂HPO₄: 10 mM
• KH₂PO₄: 1.8 mM
• Tween^® 20 detergent: 0.1% (w/v)

PBST Recipe Calculator

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• 10X Tris-Buffered Saline (TBS) Stock Solution
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X PBS (Phosphate-Buffered Saline) 
• Non-Fat Dry Milk (NFDM) Blocking Buffer
• Bovine Serum Albumin (BSA) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions