The ProteoPrep Reduction & Alkylation Kit contains the necessary reagents to conveniently reduce and alkylate disulfide binds in preparation for 2D gel electrophoresis. These reagents are compatible with chaotropic extraction reagents and are conveneintly packaged to save time and increase efficiency. The kit contains five vials of both the reducing agent, tributylphosphine (TBP), and the alkylating agent, iodoacetamide.
Características e benefícios
Tributylphosphine is supplied safely as a 200 mM solution in N-methyl-2-pyrrolidine
Reduction and alkylation of protein samples increase 2D spot resolution
Conveniently packaged components save time and increase efficiency
Compatibility with chaotropic extraction reagents simplifies sample preparation
Informações legais
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution
Bioscience, biotechnology, and biochemistry, 84(11), 2241-2252 (2020-07-23)
A simple intracellular proteomic study was conducted to investigate the biological activities of Aspergillus niger during industrial enzyme production. A strain actively secreting a heterologous enzyme was compared to a reference strain. In total, 1824 spots on 2-D gels were
This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
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