L6893
Lipopolysaccharides (rough strains) from Escherichia coli F583 (Rd mutant)
Sinônimo(s):
LPS
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
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fonte biológica
Escherichia coli (F583 Rd mutant)
Nível de qualidade
Formulário
lyophilized powder
Impurezas
<3% Protein (Lowry)
cor
white to yellow cast
solubilidade
water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
Descrição geral
This product is phenol:chloroform:petroleum ether extracted from E. coli F 583 (Rd mutant). The source strain is from a private collection.
Aplicação
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Ações bioquímicas/fisiológicas
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Nota de preparo
Prepared by phenol-chloroform-petroleum ether extraction.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Outras notas
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 2 Oral
Código de classe de armazenamento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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The objective of this study was to evaluate the effect of smooth (S) and rough (R) forms of lipopolysaccharide (LPS) on gene expression in bovine blood neutrophils. Isolated neutrophils (10(7) cells/mL) were treated with Escherichia coli LPS serotype O111:B4 (S+R)
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PloS one, 12(2), e0170346-e0170346 (2017-02-09)
Occludin is a key tight junction (TJ) protein in cerebral endothelial cells (CECs) playing an important role in modulating blood-brain barrier (BBB) functions. This protein (65kDa) has been shown to engage in many signaling pathways and phosphorylation by both tyrosine
Chandan Singh et al.
Molecular microbiology, 114(3), 510-520 (2020-05-29)
The outer membrane is a key virulence determinant of gram-negative bacteria. In Yersinia pestis, the deadly agent that causes plague, the protein Ail and lipopolysaccharide (LPS)6 enhance lethality by promoting resistance to human innate immunity and antibiotics, enabling bacteria to
J S Nielsen et al.
Scandinavian journal of immunology, 75(2), 193-202 (2011-08-23)
Immunosuppression induced by lymphocyte apoptosis is considered an important factor in the pathogenesis of sepsis and has been demonstrated in both animal models of lipopolysaccharide (LPS)-induced endotoxemia and septic patients. As rough-form LPS (R-LPS) has recently been shown to elicit
Iga Kucharska et al.
Biochemistry, 55(36), 5061-5072 (2016-08-18)
Pseudomonas aeruginosa is an opportunistic human pathogen causing pneumonias that are particularly severe in cystic fibrosis and immunocompromised patients. The outer membrane (OM) of P. aeruginosa is much less permeable to nutrients and other chemical compounds than that of Escherichia
Artigos
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
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