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FLE250

Firefly Lantern Extract

Name: Firefly Lantern Extract
Brand: SIGMA

for ATP determination

Sinônimo(s):

FLE

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Sobre este item

NACRES:

NA.81

UNSPSC Code:

12352202

Form:

solution

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Propriedades

Quality Segment

200

form

solution

packaging

pkg of 250 mg dried lanterns

technique(s)

protein detection: suitable, toxicology assay: suitable

storage temp.

−20°C

Descrição

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Application

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg²⁺ to yield a light-emitter oxyluciferin.

Preparation Note

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO₄^.

Other Notes

Crude luciferin and luciferase

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Este Item	SCT154	SCT150	SCT151
Sigma-Aldrich FLE250 Firefly Lantern Extract	Sigma-Aldrich SCT154 Firefly Luciferase Assay	Sigma-Aldrich SCT150 Firefly Luciferase HTS Assay	Sigma-Aldrich SCT151 Ensaio de luciferase Firefly (liofilizado)
description for ATP determination	description Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.	description Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours	description Lyophilized flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.
technique(s) toxicology assay: suitable	technique(s) cell based assay: suitable	technique(s) cell based assay: suitable	technique(s) cell based assay: suitable
form solution	form -	form -	form -
storage temp. −20°C	storage temp. -65 to -96°C	storage temp. -65 to -96°C	storage temp. -10 to -25°C
Quality Level 200	Quality Level -	Quality Level -	Quality Level -
packaging pkg of 250 mg dried lanterns	packaging -	packaging -	packaging -

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Classe de armazenamento

11 - Combustible Solids

O que está acontecendo?

WGK 3

Ponto de inflamação (°F)

Not applicable

Ponto de inflamação (°C)

Not applicable

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