FD40S

Isotiocianato de fluoresceína–dextrana

average mol wt 40,000

• Sinônimo(s): FITC–Dextrana
• Número CAS: 60842-46-8
• Número MDL: MFCD00131092
• Código UNSPSC: 12352201
• NACRES: NA.25

Propriedades

• fonte biológica: synthetic (organic)
• conjugado: FITC conjugate
• Formulário: powder
• peso molecular: average mol wt 40,000
• Extensão da rotulagem: 0.003-0.020 mol FITC per mol glucose
• cor: yellow to dark orange
• solubilidade: H₂O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.[1][2] Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.[3] FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9. [4]

Aplicação

Dextrana marcada com isotiocianato de fluoresceína para possível uso em estudos de perfusão em animais.

Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:

• Fluorescein-dextran microscopy.[5][6][7]
• To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.[8]
• To quantify endocytic activity by flow cytometry.[9]
• To label α-lactalbumin and ribonuclease.[10]

FITC-dextran is suitable for extensive microcirculation and cell permeability research utilizing microfluorimetry. It is also suitable for the study of plant cell wall porosity and capillary permeability.

Ligação

Similar to FD-40, but produced by Sigma.

Nota de preparo

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Outras notas

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315,H319,H335
• Declarações de precaução: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)