It is recommended to reconstitute Product No. C3142 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme.
C3142
α-Chymotrypsin from bovine pancreas
(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Sinônimo(s):
TLCK-Chymotrypsin
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Sobre este item
Número CAS:
UNSPSC Code:
12352204
eCl@ss:
42010112
EC Number:
232-671-2
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
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type
Type VII
form
essentially salt-free, lyophilized powder
specific activity
≥40 units/mg protein
mol wt
25 kDa
solubility
1 mM HCl: soluble 10 mg/mL, clear
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... CTRB1(618826)
Application
α-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.[1]
Biochem/physiol Actions
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Preparation Note
TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.
Analysis Note
Protein determined by A1%/280
Other Notes
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
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Este Item | |||
|---|---|---|---|
| specific activity ≥40 units/mg protein | specific activity ≥40 units/mg protein | specific activity ≥40 units/mg protein | specific activity ≥40 units/mg solid |
| Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) |
| form essentially salt-free, lyophilized powder | form solid | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility 1 mM HCl: soluble 10 mg/mL, clear | solubility - | solubility 1 mM HCl: soluble 2.0 mg/mL, clear | solubility 1 mM HCl: soluble 10 mg/mL, clear, colorless |
| mol wt 25 kDa | mol wt 25 kDa | mol wt 25 kDa | mol wt 25,656 Da by calculation |
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signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de armazenamento
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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How do you recommend preparing a solution of Product No. C3142?
1 answer-
Helpful?
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What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C3142)?
1 answer-
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.This information and the basic structure description of the enzyme can be found on our product information sheet (under Documents, above) at our website.
Helpful?
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How can solutions of alpha-chymotrypsin (Product No. C3142) be stored?
1 answer-
Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the pH optimum for the enzyme, Product No. C3142?
1 answer-
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
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