62310
Lipase from Rhizopus niveus
powder (fine), ≥1.5 U/mg
Sinônimo(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
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| Para você/SKU | Disponibilidade | Preço |
|---|---|---|
10 g | Verificar disponibilidade no carrinho | R$ 261,00 |
50 g | Verificar disponibilidade no carrinho | R$ 718,00 |
Sobre este item
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.26
EC Number:
232-619-9
MDL number:
Specific activity:
≥1.5 U/mg
Biological source:
fungus (Rhizopus niveus)
Assistência Técnica
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Estamos aqui para ajudarbiological source
fungus (Rhizopus niveus)
Quality Segment
form
powder (fine)
specific activity
≥1.5 U/mg
mol wt
Mr ~83000
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
General description
Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.
Application
Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.
Biochem/physiol Actions
Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Other Notes
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)
1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.
Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
1 of 1
Este Item | |||
|---|---|---|---|
| specific activity ≥1.5 U/mg | specific activity ≥30 U/mg | specific activity ≥2 U/mg | specific activity 15-25 U/mg |
| biological source fungus (Rhizopus niveus) | biological source fungus (Rhizopus oryzae) | biological source (from Candida rugosa) | biological source fungus (Candida rugosa) |
| form powder (fine) | form powder | form powder | form lyophilized solid, powder (fine) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt Mr ~83000 | mol wt - | mol wt - | mol wt - |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de armazenamento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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