62310

Lipase from Rhizopus niveus

powder (fine), ≥1.5 U/mg

• Sinônimo(s): Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Número CAS: 9001-62-1
• Número da licença da enzima: 3.1.1.3 (BRENDA, IUBMB)
• Número CE: 232-619-9
• Número MDL: MFCD00131509
• Código UNSPSC: 12352204
• NACRES: NA.26

Propriedades

• fonte biológica: fungus (Rhizopus niveus)
• Nível de qualidade: 100
• forma: powder (fine)
• atividade específica: ≥1.5 U/mg
• peso molecular: M_r ~83000
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• chave InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Descrição

Descrição geral

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Aplicação

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Ações bioquímicas/fisiológicas

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Definição da unidade

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Outras notas

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)